Use of bacterially expressed dsRNA to downregulate Entamoeba histolytica gene expression.
BACKGROUND:Modern RNA interference (RNAi) methodologies using small interfering RNA (siRNA) oligonucleotide duplexes or episomally synthesized hairpin RNA are valuable tools for the analysis of gene function in the protozoan parasite Entamoeba histolytica.However, these approaches still require time-consuming procedures including transfection and d